Post-amplification
products
were
analyzed
on
the
ABI Prism 7500 Sequence Detection System using the allelic discrimination option, and genotype calls were determined manually by comparing
to six No Template Controls (Grandy, Zhang, & Civello, 1993). The DRD2
rs1799732 [ − 141delC] was genotyped in the same manner using a custom designed TaqMan assay [forward primer: 5′ CAA AAC AAG GGA
TGG CGG AAT C; reverse primer 5′ CCA CCA AAG GAG CTG TAC CT; reporter 1 sequence (VIC): 5′ TAC CCG TTC CAG GCC G; reporter 2 sequence
(FAM): 5′ CTA CCC GTT CAG GCC G].