2.7. Standards
Vitamin D2 stock solution: 5.0 mg vitamin D2 standard was
weighed and dissolved in methanol and transferred to a 10 ml
low actinic volumetric flask and volume was made up with methanol.
Vitamin D2 stock solution was diluted to prepare standards
having concentration of 1, 2, 3, 4 and 5 ng per 100 ll injection