Interleukin-1b (CV¼7.7%), interleukin-6 (CV¼5.1%), interleukin-10 (CV¼9.9%), and myoglobin
(CV¼7.3%) were assessed by chemiluminescence-immunometric assay (Immulite, DPC, USA).
Fatty acid binding protein (CV¼2.4%) was measured by an enzyme-linked immunosorbant assay
(Hycult Biotechnology, Uden, Netherlands) and lactate dehydrogenase (CV¼0.8%) and plasma
creatine kinase (CV¼0.6%) activity were determined using an enzymatic assay (Roche Diagnostics,
Mannheim).