The majority of studies addressing gender differences focused mainly on emotion recognition from facial expression, and very few studies have examined the potential contributions of individual differences to trustworthiness judgment of an unfamiliar, emotionally neutral faces. A previous study showed that demographic variables, including gender, do not seem to influence judgments of trustworthiness and approachability [30]. However, there is some electrophysiological evidence suggesting that there may be differences in the way facial trustworthiness is processed by women and men [42]. Moreover, a recent study [43] showed different adaptation effects on perception of face trustworthiness (i.e. visual aftereffects) for women and men. Consistent with these studies, our findings suggest that gender is a factor that should be considered in studies on first impressions. Critically, however, the obtained effects are rather small and, consequently, caution is required in interpreting gender effects.