2.8. Statistical analysis
Prior to performing statistical analyses, the normal distribution
of the data was checked and confirmed using the ShapiroeWilk
test. The data are presented as the mean standard deviation (St.
dev.) of the results from three trout. An analysis of variance
(factorial ANOVAs) was run to determine whether the differentially
expressed gene differed between the replicates for each individual
gene, followed by Tukey’s multiple comparison test to assess the
differences between the vaccinated and control group. The Student
t-test was also used to compare some paired samples. All statistics
were analysed with the IBM SPSS Statistics package, an integrated
family of products that addresses the entire analytical process, from
planning and data collection to analysis, reporting and deployment