Immobilized Clostridium butyricum TISTR 1032 on sugarcane bagasse improved hydrogen
production rate (HPR) approximately 1.2 times in comparison to free cells. The optimum
conditions for hydrogen production by immobilized C. butyricum were initial pH 6.5 and
initial sucrose concentration of 25 g COD/L. The maximum HPR and hydrogen yield (HY) of
3.11 L H2/L substrate$d and 1.34 mol H2/mol hexose consumed, respectively, were obtained.
Results from repeated batch fermentation indicated that the highest HPR of 3.5 L H2/L
substrate$d and the highest HY of 1.52 mol H2/mol hexose consumed were obtained at the
medium replacement ratio of 75% and 50% respectively. The major soluble metabolites in
both batch and repeated batch fermentation were butyric and acetic acids.
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