Feeding marks
In a separate experiment, the feeding marks were observed
following the method of Natio (1964). To quantify the
role of insects while feeding on different genotypes, a
pair of newly emerged adults starved for 1 h was confined
in mylar cage on 30-day-old caged uninfested plants of
each tested genotype. The feeding marks were observed
under microscope after 24 h of feeding by removing
the plants from the pots and treated with 0.1%
Rhodamine B Analytical Reagent dye for 15 min.