Fig. 3A–E shows chromatograms resulting from the analysis of
various pomegranate products, including commercial drinks and
handmade juice. Concentrations of the targeted metabolites found
in the samples are listed in Table 2. There is a high degree of similarity
between the chromatograms obtained for the 100% pomegranate
juice drinks (POM Wonderful (Fig. 3C), All Natural
(Fig. 3D), and Langers 100% (Fig. 3E)) and pomegranate marc
extract (Fig. 2A). Whereas the chromatograms resulting from the
fresh hand-squeezed juice (Fig. 3A) and pomegranate containing
drink (Fig. 3B, Honest Ade Pomegranate Blue) were dominated by
different peaks and contained minimal concentrations of the targeted
phenolics (Table 2).
The chromatogram resulting from the analysis of handmade
juice from pomegranate arils showed two high response peaks
appearing at 10.11 and 11.44 min. Evaluating the UV–VIS spectrum
of these peaks, we tentatively identified the peak at 11.44 min as
cyanidin 3,5-diglucoside (kmax 517, 279 nm), but were unable to
assign an identity to the other peak (10.11 min, kmax 265 nm).
Analysis of the chromatogram at 510 nm (data not shown) also revealed
the presence of other anthocyanins, but additional efforts to
identify these compounds were not undertaken because it was
outside of the scope of our current objectives. Most reports found
Fig. 3A–E shows chromatograms resulting from the analysis of
various pomegranate products, including commercial drinks and
handmade juice. Concentrations of the targeted metabolites found
in the samples are listed in Table 2. There is a high degree of similarity
between the chromatograms obtained for the 100% pomegranate
juice drinks (POM Wonderful (Fig. 3C), All Natural
(Fig. 3D), and Langers 100% (Fig. 3E)) and pomegranate marc
extract (Fig. 2A). Whereas the chromatograms resulting from the
fresh hand-squeezed juice (Fig. 3A) and pomegranate containing
drink (Fig. 3B, Honest Ade Pomegranate Blue) were dominated by
different peaks and contained minimal concentrations of the targeted
phenolics (Table 2).
The chromatogram resulting from the analysis of handmade
juice from pomegranate arils showed two high response peaks
appearing at 10.11 and 11.44 min. Evaluating the UV–VIS spectrum
of these peaks, we tentatively identified the peak at 11.44 min as
cyanidin 3,5-diglucoside (kmax 517, 279 nm), but were unable to
assign an identity to the other peak (10.11 min, kmax 265 nm).
Analysis of the chromatogram at 510 nm (data not shown) also revealed
the presence of other anthocyanins, but additional efforts to
identify these compounds were not undertaken because it was
outside of the scope of our current objectives. Most reports found
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