2.3. Preparation and measurement of injera texture
Injera was prepared according to Yetneberk et al. (2004) with
modification. To initiate the second fermentation, 0.5 g commercial
instant dried baker’s yeast and 1.5 g sugar was added to the rest of
the fermented batter and stirred thoroughly. The yeast fermented
batter (20 g)was weighed into a 90mmplastic Petri dish and baked
in a 900 Watt microwave oven (for 45 s) until it formed a honeycombed
structured surface.
The texture of injera was evaluated using a protocol similar to
that of Yetneberk et al. (2004). Each injera was put into a separate
ziplock-type polythene bag and stored at 25 C in an incubator for
1 h, 24 h and 48 h. For texture analysis, each injera was cut using
a 65 mm diameter cookie cutter while still fresh, to obtain
a uniform sample size. The texture of each type of injera were
analysed three times using a TA-XT2 Texture Analyser (Stable Micro
Systems, Godalming, UK). The stiffness was measured as maximum
bending force determined using a three-point bend rig with an
aluminium bar (5 mm thick and 90 mm long) attachment, and the
two adjustable supports of rig base plate were set 30mmapart. The
testing profilewas set as follows: Pre-Test Speedwas 1.0 mm/s; Test
Speed was 3.0 mm/s; Post-Test Speed was 10.0 mm/s; Distance was
5 mm; Trigger type was 0.049 N. Three pieces of injera per treatment
were analysed.