and glucose may be 10
mg/100 mL or lower. A wet-mount of the CSF should be
microscopically examined immediately after collection,
preferably equipped with phase-contrast optics, for the
presence of actively moving trophozoites followed by
specific immunofluorescent staining
(67)
. Since Naegleria
feed upon bacteria, they can be isolated from clinical
specimens such as brain tissue and CSF using non-nutrient agar spread with bacteria. Naegleria grow s well
on monolayers of Vero monkey kidney cells ( E6) and
human lung fibroblasts (HLF) cell cultures, destroying
the confluent layers within 2-3 days. Like Acanthamoeba
and Balamuthia, N. fowleri can be grown in a cell-free axenic medium as well as in a chemically defined
medium