The observed Scheme 1. Synthesis of the EHEC Functionalized with BM,
Showing Its Protonated (3a) and Deprotonated Form (3b) ppearance of the solvatochromic band at λmax = 444 nm.
Interestingly, the deprotonation of the dye units in the film leads to the appearance of the solvatochromic band of the dye atλmax= 468 nm. This bathochromic shift ofΔλmax= +24 nm
suggests that the dye is in a less polar environment in the film.
28,31