Well, the PH of medium, pretreat to explants (scuffing, cool or heat treat, etc.) and culture condition (temperature, humidity, illumination etc.) are also important in safflower tissue culture. Generally, selecting 5-7 day old seedlings as explants source, MS as basal medium, sucrose as carbon source is favorable for safflower tissue culture. The PH of medium was adjusted to 5.8 and the culture condition was set as (25 ± 3)℃, 16-h photoperiod with irradiance of 30 μmol · m-2 · s-1 (cool white fluorescent lamps).