A co-culture of MZA-3 andS. cerevisiae; and a monoculture ofS. cerevisiaeand MZA-3 enzyme (both supplemented with glucoamylase) resulted into bioethanol yield (percentage of the theoretical yield) of 91 and 95 at efficiency (percentage) of 84 and 96, respectively. Direct bioconversion of raw starch to bioethanol was achieved at 30 C through the co-culture approach. This could be attractive since it may significantly downsize energy expenses