Another alternative to combine heat and HPH was the previous thermal treatment to promote the spores germination and posterior homogenization to inactivate the germinated spores.Again,no significant reductions of B. stearothermophilus and C.sporogenes were observed,indicatingthat HPH up to 300MPa was also not able to inactivate previous germinated spores .Considering that the combination of heat and homogenization did not presented satisfactory results,it was evaluated the effect of homogenization at high temperature in the spores.The results again did not indicated efficacy on spores inactivation.The last alternative to makes viable the use of HPH to inactivate B.stearothermophilus and C.sporogenus spores was the multiple treatments at 300 MPa .The results of this test for B.stearothermophilus spores inactivation are shown in figurel.