Aliquots from each ejaculate (10 ml) were diluted
1:20 in an extender (Tris-citrate acid-glucose) containing
bovine serum albumin 0.3% (BSA) to prevent the
spermatozoa from sticking to the glassware during
motility analysis. Ten microliters of the diluted sample
was placed into a 10 mmdeep Makler counting chamber
(Sefi Medical Instruments, Haifa, Israel) for motility
analysis using a computer-assisted sperm analysis
(CASA) system (Sperm Class Analyzer, S.C.A.,
Microptic, Barcelona, Spain). Sperm motility was
assessed at 37 8C with 10X phase contrast objective.