VLC fractions 6–8 were active against S.
aureus SA-1199B at 64 mg/L, had similar TLC profiles and were
pooled (total of 842.0 mg). This combined fraction was separated by
Sephadex LH-20 chromatography giving five fractions eluted with chloroform:methanol
(1:1) and one fraction eluted with methanol. The fraction eluted with methanol (80.9 mg) was active at a MIC of 1 mg/L