Bacterial strains were grown on agar plates at 37C overnight under aerobic conditions and bacterial cells were collected in
10 mL of phosphate buffered saline using a sterile 10 mL loop under a biosafety cabinet. The optical density of the bacterial suspension was adjusted to an absorbance value of 0.10 at 600 nm on a Beckman DU640 spectrophotometer
. A 100 mL aliquot of the bacterial suspension was added to the wells of a 6-well culture plate containing either 5 mL of skim milk, blueberry juice alone, blueberry juice supplemented with 5% glucose or 50% blueberry juice and 50% skim milk mixture and incubated for 24, 48 and 72 h at 37C. For determination of time required for inhibition of growth, 100 mL aliquot of the bacterial suspension was added to the wells of a 6-well culture plate containing either 5 mL of BHI broth , blueberry juice alone, blueberry juice supplemented with 5% glucose or 50% skim milk plus 50% blueberry juice and incubated for 0, 1, 2, 4 or 6 h at 37C. Following incubation, serial dilutions were prepared in PBS and plated on strain-specific agar to measure bacterial growth
Bacterial strains were grown on agar plates at 37C overnight under aerobic conditions and bacterial cells were collected in10 mL of phosphate buffered saline using a sterile 10 mL loop under a biosafety cabinet. The optical density of the bacterial suspension was adjusted to an absorbance value of 0.10 at 600 nm on a Beckman DU640 spectrophotometer. A 100 mL aliquot of the bacterial suspension was added to the wells of a 6-well culture plate containing either 5 mL of skim milk, blueberry juice alone, blueberry juice supplemented with 5% glucose or 50% blueberry juice and 50% skim milk mixture and incubated for 24, 48 and 72 h at 37C. For determination of time required for inhibition of growth, 100 mL aliquot of the bacterial suspension was added to the wells of a 6-well culture plate containing either 5 mL of BHI broth , blueberry juice alone, blueberry juice supplemented with 5% glucose or 50% skim milk plus 50% blueberry juice and incubated for 0, 1, 2, 4 or 6 h at 37C. Following incubation, serial dilutions were prepared in PBS and plated on strain-specific agar to measure bacterial growth
การแปล กรุณารอสักครู่..
