These sequences were used to design and synthesize several sets of primers to specifically amplify each of the three serotypes of poliovirus,then each primer pair (forward and reverse) was used to run RT-PCR for identification of polioviruses under different annealing temperatures (melting temperature ±4–10 ◦C),and only the ones that under optimum annealing temperature did not produce non-specific amplification products were selected to perform this work and are presented in Table 1.The primers were synthesized by Integrated DNA Technologies, Inc.