Peptide mass fingerprinting (PMF) of the molecule
To obtain the PMF of Racotumomab ST produced from both
scales, were taken, reduced and carboxymethylated with the aim of
separating and denaturing the light and heavy chains. This is after
digestion was performed with trypsin (Promega, USA) as described
by Wang et al. [27]. Tryptic peptides were desalted by using the
extraction solid phase Cleanup C18 Pipette Tips recommended by
the manufacturer.