Seed cultures were prepared by inoculating 2 ml spore suspension (108 spores/ml) to 100 ml inoculum medium and they were incubated at 30°C and 200 rpm for 48 h.
Seed cultures were prepared by inoculating 2 ml spore suspension (108 spores/ml) to 100 ml inoculum medium and they were incubated at 30°C and 200 rpm for 48 h.