Yeast was also cultured in the liquor from steam exploded
duckweed (which contains water-soluble sugars) to investigate
the adaptability of yeast to SE medium. The SE medium was prepared
as follows: a range of SE slurries (150, 170, 190, 210 and
230 C) were centrifuged and supernatants were transferred into
sealed bottles that were subsequently autoclaved and then cooled
before yeast solution was added. With 10% (v/v) yeast inoculation,
yeast was grown in the SE medium for 2 days.