The antimicrobial activities of chito-oligosaccharides against four Gram-positive and seven Gramnegative bacteria were compared to chitosan and chitin with an emphasis on the effects of biopolymer
molecular weight (Mv) and degree of deacetylation (DD). Chitin was isolated from shrimp (Parapenaeus
longirostris) shell waste by sequential chemical treatments. Chitosan and its oligomers N-acetyl chitooligosaccharides and chito-oligosaccharides were prepared by deacetylation and chemical hydrolysis,
respectively. Chitin exhibited a bacteriostatic effect on Gram-negative bacteria, Escherichia coliATCC
25922,Vibrio cholerae, Shigella dysenteriae, and Bacteroides fragilis. Chitosan exhibited a bacteriostatic
effect on all bacteria tested, exceptSalmonella typhimurium. The oligomers exhibited a bactericidal effect
on all bacteria tested.