Principles of the Procedure
Nitrogen, vitamin, and carbon sources are provided by Enzymatic Digest of Casein and Enzymatic Digest of
Animal Tissue in Blood Agar Base, Improved. Yeast Extract is a vitamin source. Corn Starch is added to
ensure any toxic metabolites produced are absorbed, and enhance organism growth.5
Sodium Chloride
maintains the osmotic balance of the medium. Agar is the solidifying agent.