2.3 Bacterial cellulose yield
The synthesized cellulose was separated from the medium by filtration and boiled in 1% sodium hydroxide solution for 30 min to remove cells and medium embedded in the cellulose material,then rinsed 3 days with deionized water to reach neutral pH (Yanet al., 2008). The BC samples were freeze-dried to obtain dry mate-rial. Bacterial cellulose yield was calculated from the dry weight ofpurified cellulose.