FIG. 2. Influence of reaction time on the esterification degree (ED) of free fatty acids
(FFAs) from used vegetable oil (UVO) (closed circles) and the microalgal biomass from
N. gaditana (open squares) catalysed by Novozym 435 at large scale (scaling factors of
10 and 3.6). Operational conditions for FFAs from UVO: 40 g FFAs, 8.6 mL methanol
(methanol/FFA molar ratio 1.5:1), 4 g Novozym 435 (Novozym 435/FFA ratio 0.1:1 w/w),
200 rpm and 40C. For FFAs from microalgal biomass: 14.3 g FFAs, 3.08 mL methanol
(molar ratiomethanol/FFA1.5:1),1.43 gNovozym435 (Novozym435/FFAratio 0.1:1w/w),
200 rpm and 40C