Highlights
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Different bacterial species isolated from predigester of Nisargruna Biogas Plants.
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Bacterial isolates were identified by biochemical tests and 16s rDNA sequencing.
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FTIR spectra and its 1st order derivative proved useful in bacterial identification.
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FTIR more conclusive in differentiating genetically close organisms B. cereus and B. thuringiensis.
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FTIR, NIR and UV–Vis together made spectroscopic approach of identification of bacteria very robust and reliable.