the use of natural product as anticancer agents has a long history including traditional medicines and several phytochemical-derived drugs currently used in chemotherapy over 50% of the drugs in clinical trials for anticancer activity were isolated from natural sources in asian countries,herbal formulations from a mixture of plants are often used by traditional indian medicine has been consumed for various tumor prevention or suppression.phytotherapy is considered as an alternative to reduce the adverse effect on the use of synthetic drug. in the recent study of thai medicinal plants, Dioscorea membranacea contained dioscorea lide B and dioscorea moonee, which were potent antiproliferation againt the breast adenocarcinoma MCF-7. Mucuna colleettii was potent antiproliferation aganinst MCF-7. The study on the essentail oil from the thai medicinal plants, psidiom guajava L.leaf and Ocimum basilicum L. showed anti-proliferratiive activity against human mouth epidermal carcinoma KB cells and murrine leukmia P388 cells. Polyphenol and sterol group from tea exhibited anti-proliferation against lung cancer cell. Flavononoids from Brazillian propolis inhibited the growth of lung cancer cell. thee stem extracts of euphorbia antiquorum,the bark extracts of croton obloongifoli ,the root extracts of kaempferia parviflora and the frower extracts of melodorum fruticosum have potential antiproliferation against lung cancer cells (chago). this is definitely very low numbers of tested plants. We,therefore make a study in the 10 thai rejuvenating plants against the proliferation of CHAGO cells.