respectively (Bertoft et al., 2012a).Changes were observed in the number of b0-chains in clusters isolated from A- and B-type granules. In addition, a slight increase of b1a-chains in clusters of A-type granules was observed at 28 DAA.In contrast, the other b-chain categories, especially b2- and b3-chains, remained practically unchanged during both A- and B-typegranule development for two plausible reasons: (1) inside clusterslong b-chains remained unaffected during granule development,and glucan chain rearrangement (trimming) occurred primarilywith short b-chains; (2) even though structural changes occurred in the long B-chains of AP, the clusters did not reflect these changesdue to the removal of the affected segments during isolation of the clusters.