A total of 1710 cell aggregates of Xushu18 (Fig. 2A) cocultivated
with the A. tumefaciens strain EHA 105 were cultured on the selective
medium with 2.0 mg/l 2,4-D, 100 mg/l Carb and 7mg/l Hyg.
Eight weeks after selection, 488 Hyg-resistant embryogenic calluses
were produced from them (Fig. 2B)