3.4. Lipase production by mixed substrate fermentation
A novel mixed substrate from soapstock, fatty acids residues and olive oil was prepared to enhance the lipase production. Fig. 4b shows the time course of lipase production using the mixed substrate (concentration is not mentioned) and the results were 10 U/ml and 0.3mg/ml for lipase activity and protein content respectively, which is considered higher than the activities and yields of that obtained by soapstock and fatty acids residues individually. Fig. 5a shows the
maximum activity of lipase produced using mixed substrate compared with different maximum activities obtained using pitch (fatty acids residues), olive oil and soapstock individually. The nutrients and trace elements present in a mixed medium enable the
organism to yield more protein favorably by influencing its
biochemical pathway for lipase production (Cordova et al., 1998).