The nuclear and chloroplast DNA sequence is useful in examining the genetic variation of complex species at the inter-specific level, and the proposed phylogenetic relationship among Memecylon species based on nuclear, chloroplast DNA sequence and Jaccard dissimilarity coefficient values is convincing. Therefore, further work has to be carried out using ITS, matK and rbcL markers in combination including the Memecylon species of Western Ghats and compare them with rest of the deposited sequences of other Memecylon species from different parts of the world.