Materials and methods
Healthy and witches’ broom diseased lime (Citrus aurantifoliaChristm) plants were collected from key limegardens of Jiroft in the south of Iran.Single node shoots were excised from mother plans and surface-sterilized by soaking in a solution containing 6% bleach and 1% tween 20 for 20 min. The explantswere rinsed tree times in sterile distilled water