(4) Superoxide dismutase
Superoxide dismutase catalyzes the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are important antioxidant defense systems in nearly all cells exposed to oxygen. O2 * gives rise to more reactive oxygen species with a higher potential toxicity in most organic compounds. SOD protects tissues from membrane lipid destruction. The level of SOD is reduced in hepatotoxicity while elevated enzyme levels indicate hepatic protection. Because of the instability of its substrate, O2*, all available assays of SOD are indirect and depend on its ability to scavenge O2* from reaction mixtures and, thus, inhibit reactions caused by O2*. One such reaction is the generation of O2* during auto-oxidation of hydroxylamine at pH 10.2 and simultaneous reduction of nitro blue tetrazolium by formation of nitrite in the presence of EDTA. The reduction of NBT is inhibited by SOD, which is measured spectrophotometrically at 560 nm [29, 34].
(4) Superoxide dismutase
Superoxide dismutase catalyzes the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are important antioxidant defense systems in nearly all cells exposed to oxygen. O2 * gives rise to more reactive oxygen species with a higher potential toxicity in most organic compounds. SOD protects tissues from membrane lipid destruction. The level of SOD is reduced in hepatotoxicity while elevated enzyme levels indicate hepatic protection. Because of the instability of its substrate, O2*, all available assays of SOD are indirect and depend on its ability to scavenge O2* from reaction mixtures and, thus, inhibit reactions caused by O2*. One such reaction is the generation of O2* during auto-oxidation of hydroxylamine at pH 10.2 and simultaneous reduction of nitro blue tetrazolium by formation of nitrite in the presence of EDTA. The reduction of NBT is inhibited by SOD, which is measured spectrophotometrically at 560 nm [29, 34].
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