In forensic science practice, detecting the causative agent of an
explosion in body fluid is very important when victims are found
at an explosion scene. We experienced an actual acetylene explo-
sion case, and it was necessary to analyze acetylene in blood and
urine immediately. Previously, the detection of acetylene in the
lungs by gas chromatography–mass spectrometry (GC–MS) with
the headspace method was reported as a case report[4]; however, in
that report, Williams et al.[4] considered that the analytical method
was unreliable for quantitative analysis from blood. Few reliable
methods have been reported to quantify acetylene in biological
samples. We therefore investigated rapid and reliable methods for
measuring acetylene in blood and urine.