Antimicrobial activity of the films was tested by the method of Otoni et al. (2014) with some modifications. Escherichia Coli and Sta- phylococcus aureus were inoculated in nutrient agar medium. Spread plates of culture medium were inoculated with 0.2 mL of inoculums containing 106 CFU/mL of each microorganism with 24 h growth. The 12 mm diameter disk cut from PPF film with or without was placed on the respective culture medium. The bacterial strains were incubated at 37 °C for 24 h. The inhibition zones were measured with a caliper and recorded in millimeters. All tests were performed in triplicates.