The desalted peptide was mixed 1:1 with -
cyano-4-hidroxicinnamico acid (CHCA), which is then applied onto
a MALDI plate and allowed to dry at room temperature. For PMF
analysis spectrometer was operated in the reflectron mode, m/z
range 700–3500, the laser power was 70 and 100 sample profiles
were acquired. For each profile there was an average of 20
laser shots. For calibration PeptideMass Calibration Kit (Sigma, Cat.
No-1KT MSCAL2) was used. The spectra were processed with the
program Axima Biotech Launchpad (Shimadzu, Japan).