The serological
tests were carried out subsequent to return to
the United Kingdom. All sera were initially screened for
hepatitis B antigenemia. The 11 positive samples were
analyzed for malaria antibodies but not for the other
serological parameters. Immunoghobuhins IgA, IgG, and
1gM were assayed using immunodiffusion with standard Mancini plates (Hoechst). The serum albumin was assayed
by the Bromocresoh green technique. Total serum
IgE was assayed by the double antibody technique (13).
The malaria antibody concentrations were assayed by
the ELISA technique for Plasmodium falciparum anti- bodies (14). The following autoantibody tests were performed:
thyroid microsomal (Wehlcome; Thymune, M.);
thyroid cohhoid (15); smooth muscle antibody (16); antinuclear
factor (17); parietal cell antibody (18); reticuhin
antibody (19); mitochondrial antibody (20); R.A. Latex
test (21); Paul-Bunnell with sheep cells (22) and horse
cells (23).