chemicals like formaldehyde and phenol are proved to adversely
affect virus antigenicity. Thus, it is necessary to find replacement
inactivating agents which are not expensive, safe and available [1].
Hydrogen peroxide (H2O2) is a strong oxidizing agent. Many
studies investigated its ability to preserve antigenic epitopes and
other studies used it as inactivating agent for development of viral
vaccines against both DNA and RNA viruses including lymphocytic
choriomeningitis virus (LCMV), yellow fever virus (YFV), West
Nile virus (WNV), Vaccinia virus (VV) and monkeypox virus (MPV)
[8–10].
Keeping this in mind, our study aimed to investigate the capability
of H2O2 in causing a complete inactivation of fixed rabies virus
without adversely affecting its antigenicity and immunogenicity.