The cultures obtained in blood agar colonies were stained
in Gram (Golden Bell Reactives TM DMACA TM, Cat. 261181)
and indole (BBL TM DMACA TM, Cat. 261187) and biochemical
tests for bacterial identification (BD BBL TM Crystal Identifi-
cation System Enteric/Non-fermenter ID Kit, Cat. 245000).
The oxidase test was performed as the following: three
drops of the reagent were added to the Whatman filter paper
number 1 in sterile conditions and 3---4 pure colonies were
pleated over it. After 10---30 s the reading was performed
and the results were interpreted: the violet color or purple
color as a positive and without a change as a negative.
The indole test was achieved as the following: 3 drops of
a reagent were deposited in Whatman filter paper number
1 in sterile conditions and 3---4 pure colonies were pleated
over it. The reading took place after 2 min and the results
interpreted: blue-green color as a positive and pink or no
change of color as a negative.
For the biochemical identification test, the pure colonies
obtained in blood agar were taken and then deposited in
one tube. The inoculums were adjusted to one scale of
Mac Farland 1.0 in Nephelometer (BBL TM Crystal Spec tm
Nephetometer Cat. 245009). The bacterial suspension was