hich blood is collected, for example feeding or fasting, the anaesthetic used, whether
the animals are bled randomly and the site of blood collection. For these reasons, it is
advisable that the blood should be collected in the same manner at each sampling time in
order to minimize variation and that the volume of blood collected should be restricted to
1.5ml or 1% of body mass, whichever is the smallest. The OECD Shadow Toxicology
Group did not see a need for fasting animals prior to the collection of blood for clinical
chemistry measurements in a routine screening approach. Rather, it was felt that the
measurement of non-fasting glucose was a more sensitive measure than fasting glucose.
However, the importance of fasting for a more in-depth investigation of a particular effect
on carbohydrate or lipid metabolism was recognized
hich blood is collected, for example feeding or fasting, the anaesthetic used, whetherthe animals are bled randomly and the site of blood collection. For these reasons, it isadvisable that the blood should be collected in the same manner at each sampling time inorder to minimize variation and that the volume of blood collected should be restricted to1.5ml or 1% of body mass, whichever is the smallest. The OECD Shadow ToxicologyGroup did not see a need for fasting animals prior to the collection of blood for clinicalchemistry measurements in a routine screening approach. Rather, it was felt that themeasurement of non-fasting glucose was a more sensitive measure than fasting glucose.However, the importance of fasting for a more in-depth investigation of a particular effecton carbohydrate or lipid metabolism was recognized
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