In the present study, application of Jatropha curcas L. seed oil alone had no beneficial effects on the healing time of wounds experimentally induced in Wistar rats. However, significant effects were observed when the oil was applied simultaneously with microcurrent stimulation. In this respect, microcurrent application alone or in combination with the seed oil promoted an increase in the total number of cells, number of newly formed blood vessels, epithelial thickness, and compaction of mature collagen fibers in the wound area of animals on days 10 and 14 after experimentally induced injury.