A 2-week experiment was conducted to detect antigenic soy proteins in plasma of pigsfed soybean meal (SBM) and fermented soybean meal (FSBM). The suitability of a commercial FSBM to replace SBM in diets for newly weaned pig was also evaluated. A total of160 pigs were randomly assigned to 5 dietary treatments (n= 8): 400 g/kg soybean meal(FSBM-0), its replacement by protein from fermented soybean meal as 1/3 (FSBM-1), 2/3(FSBM-2), 3/3 (FSBM-3), and no soy protein in diet (PCON). Diets were fed at weaning,but feed intake (ADFI) and weight gain (ADG) record began after 3 days. Overall, pigs fedPCON had greater (P<0.001) performance and less diarrhea than pigs fed soy proteins. Pigsfed diets with FSBM had less (P<0.001) diarrhea than those fed the FSBM-0 diet. Westernblot analysis showed that pig plasma recognized, andsubunits of-conglycininand basic subunits of glycinin present in SBM, while acidic subunits of glycinin, and P34(human immunodominant soybean allergen) showed a weak immunoreactivity. Plasmafrom pigs fed FSBM exhibited reduced immunoreactivity towardandsubunits of-conglycinin and acidic subunits of glycinin, due to a partial hydrolysis of those proteinsduring fermentation. In agreement with that observation, analysis of soy proteins in FSBMby sodium-dodecyl sulfate-polyacrylamide gel electrophoresis showed partial digestion oflarge proteins with antigenic activity. A linear regression analysis of values from enzymelinked immunosorbent assay (ELISA) did not support the notion that anti-soy immunoreactivity in pig plasma changed significantly as the dietary inclusion of FSBM increased. TheFSBM used in this study may be used in substitution of SBM protein without detrimentaleffect on pig performance. Moreover, inclusion of FSBM in diets for newly weaned pigsmay reduce diarrhea after weaning. However, optimization of the fermentation conditionsin the commercial FSBM is still needed to produce a more hypoallergenic soybean productfor young pigs
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