As the first step of GMO analysis in enforcement laboratories, all
these DNA samples were submitted to a qPCR SYBRGreen analysis
using the PLD and t35S pCAMBIA screening markers in order to
identify potential unauthorised GMOs. As expected, all samples
containing Bt rice (100% to 0.005%) presented a positive signal,
inversely proportional to the DNA target amount, for the two
markers. Moreover, the WT rice (0%) showed only a positive signal
of the same amplitude for the PLD marker and no signal was
observed for the NTC with these screening markers