Stress indicators including the concentrations of
serum cortisol, total serum protein, blood urea nitrogen,
fasting blood sugar and PCV were measured to ascertain
whether the calcium chloride treatment was associated
with any general stress response in the experimental
animals [57]. As there were no significant alterations in
the serum concentrations of cortisol, blood urea nitrogen,
fasting blood sugar level, PCV or total serum protein
concentrations in the animals treated with calcium
chloride or surgically castrated with respect to the controls,
this method of sterilization does not appear to be
associated with any general stress response, which is
also supported by our previous findings [2,3,16,17].