In a field experiment the effects of chosen agronomical factors: method of plantation establishment, organic fertilization
with leonardite on roseroot yields and quality as well as changes in some physical properties and enzymatic
activity of two types of soil under roseroot culture were evaluated. Research have shown that leonardite
addition was the factor that affected yielding and quality parameters of plants as well as increased activity of dehydrogenases,
acid phosphatase, urease and protease, being especially effective on sandy soil. Generally, higher
yields of raw material and higher enzymatic activity were noted on loess soil than the sandy one, whereas better
plant growth was observed also in objects with autumn seed sowing. Therefore, autumn seed sowing and annual
humic substance application in the form of leonardite could be recommended in roseroot culture,whereas sandy
soil should be avoided during plantation establishment.