2.2.6. Hydrophilic and lipophilic antioxidant capacity
The method of Arnao, Cano, and Acosta (2001) was used to extract
the hydrophilic and lipophilic fractions of the seeds with
slight modifications. In brief, 1 g of SI sample was extracted first
with 15 ml of 80% methanol (hydrophilic fraction) and then with
10 ml of dichloromethane (lipophilic fraction). All the fractions
were centrifuged at 4000g for 10 min at 4 C and stored at
80 C until analysis. The lipophilic fraction was dried under nitrogen
and re-dissolved in acetone containing 7% RMDC. This sample
solution was used to measure the lipophilic antioxidant capacity
(LAC) meanwhile the hydrophilic fraction was used directly to
measure the hydrophilic antioxidant capacity (HAC).