Total polyphenol content was determined by Folin-Ciocalteu colorimetric. Leaf tissues were extracted with 80% MeOH and 50 ㎕ of extract was mixed with 1ml of 2% Na2CO3 and 50 ㎕ of 1 N Folin-Ciocalteu reagent at room temperature for 30 min. The absorbance of total polyphenol was measured at 750 nm. Gallic acid was used as standard to quantify the total polyphenol.