Organisms and culture conditions Forty-six S. thermophilus strains isolated
from dairy food products were used in this study. All the strains were collected by the
Yakult Central Institute, Tokyo (YIT), and assigned YIT numbers. In this study, all the
isolated S. thermophilus strains were represented by YIT numbers. All cultivations were
performed at 42 °C with no agitation. One loopful of dimethyl sulfoxide stock of S.
thermophilus was inoculated into 2 ml of MRS medium (Becton Dickinson, Oxford, UK).
Cells were grown overnight, after which 0.1 ml of culture broth was inoculated into
10 ml of 10% skimmed milk (Becton Dickinson). Cells were further cultured overnight.
Organisms and culture conditions Forty-six S. thermophilus strains isolatedfrom dairy food products were used in this study. All the strains were collected by theYakult Central Institute, Tokyo (YIT), and assigned YIT numbers. In this study, all theisolated S. thermophilus strains were represented by YIT numbers. All cultivations wereperformed at 42 °C with no agitation. One loopful of dimethyl sulfoxide stock of S.thermophilus was inoculated into 2 ml of MRS medium (Becton Dickinson, Oxford, UK).Cells were grown overnight, after which 0.1 ml of culture broth was inoculated into10 ml of 10% skimmed milk (Becton Dickinson). Cells were further cultured overnight.
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