this is the first time
that it was used to over-express a multiheme c-type cytochrome
from a Gram-positive bacterium in a Gram-negative bacterium.
Protein expression was assessed on SDS–page followed by
heme-staining coloration to confirm the protein-bound heme.
Fig. 1A depicts the result of protein expression tests of E. coli
JM109 (DE3) co-transformed with pEC86 and TherJR_0333 with
native signal peptide (lanes 2 and 3) and TherJR_0333 with STC
signal peptide (lanes 4 and 5) respectively