The cultures were regularly subcultured on fresh medium after 4–5 week interval. The observations were taken after every seven days of inoculation. The experiments were repeated thrice with twenty replicates per treatment. The rate of multiplication represents the number of shoots produced per explant on a specific medium after the number of days of its inoculation as mentioned in the results. The level of significance with different parameters was determined by variance (ANOVA) using SPSS software (16.0 ver.). The level of variance was analyzed by Duncan’s Multiple Range of Test (DMRT) at 0.5% level.